Journal
/
Genética
/
Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae
JoVE Revista
Genética
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Genética
Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae

Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae

5,233 Views

•

06:29 min

•

April 15, 2021

DOI:

10.3791/62131-v

DOI:
10.3791/62131-v

•

06:29 min
April 15, 2021

•

14 Views
, , , , , ,
1Department of Vector Biology,Liverpool School of Tropical Medicine, 2IMBB FORTH, 3Department of Biology,University of Crete

Summary

Automatically generated

The bipartite GAL4-UAS system is a versatile tool for modification of gene expression in a controlled spatiotemporal manner which permits functional genetic analysis in Anopheles gambiae. The procedures described for using this system are a semi-standardized cloning strategy, sexing and screening of pupae for fluorescent protein markers and embryo fixation.

Explore More Videos

GAL4-UAS SystemAnopheles GambiaeFunctional GeneticsGenetic ModificationGene ExpressionPhenotypic CharacterizationInsecticide ResistancePathogen TransmissionGenetic ControlPupae CollectionFluorescent MarkerExternal GenitaliaSexingAdult TransferBlood FeedingOviposition

Read Article

Cite this Article

Poulton, B. C., Colman, F., Anthousi, A., Grigoraki, L., Adolfi, A., Lynd, A., Lycett, G. J. Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae. J. Vis. Exp. (170), e62131, doi:10.3791/62131 (2021).

Download .ris file

Copy

Share Video

.

Copy