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Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae
JoVE Revista
Genética
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JoVE Revista Genética
Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae

Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae

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06:29 min

April 15, 2021

DOI:

06:29 min
April 15, 2021

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Summary

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The bipartite GAL4-UAS system is a versatile tool for modification of gene expression in a controlled spatiotemporal manner which permits functional genetic analysis in Anopheles gambiae. The procedures described for using this system are a semi-standardized cloning strategy, sexing and screening of pupae for fluorescent protein markers and embryo fixation.

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