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Acetilazione lisina specifica del sito degli istoni per la ricostituzione nucleosoma usando l'espansione del codice genetico in Escherichia coli
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Biochemistry
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JoVE Journal Biochemistry
Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli
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Acetilazione lisina specifica del sito degli istoni per la ricostituzione nucleosoma usando l'espansione del codice genetico in Escherichia coli

italiano

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3,837 Views

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07:26 min

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December 26, 2020

DOI:

10.3791/62113-v

DOI:
10.3791/62113-v

•

07:26 min
December 26, 2020

•

4 Views
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1Department of Chemistry,Texas A&M University

Summary

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Qui presentiamo un metodo per esprimere proteine istono acetilate usando l'espansione del codice genetico e assemblare nucleosomi ricostituiti in vitro.

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Lysine AcetylationHistonesNucleosome ReconstitutionGenetic Code ExpansionEscherichia ColiMononucleosomeEpigenetic ExperimentsBiochemical AssaysBinding AssaysStructure DeterminationHistone Lysis BufferInclusion BodiesNickel-NTA PurificationAcetylated Histone ProteinDialysisAcetic Acid Buffer

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Rowlett, C. M., Liu, W. R. Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli. J. Vis. Exp. (166), e62113, doi:10.3791/62113 (2020).

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