Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in <em>Escherichia coli</em>

Chesley  Marie Rowlett; Wenshe Ray Liu

doi:10.3791/62113

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Site specifieke Lysine acetylatie van histonen voor nucleosoom reconstitutie met behulp van genetische code uitbreiding in Escherichia coli

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Biochemistry

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JoVE Journal Biochemistry

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli

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Site specifieke Lysine acetylatie van histonen voor nucleosoom reconstitutie met behulp van genetische code uitbreiding in Escherichia coli

3,837 Views

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07:26 min

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December 26, 2020

DOI:

10.3791/62113-v

DOI:

10.3791/62113-v

•

07:26 min

December 26, 2020

•

4 Views

Chesley Marie Rowlett¹, Wenshe Ray Liu¹

¹Department of Chemistry,Texas A&M University

Summary

Hier presenteren we een methode om geacetyleerde histoneiwitten uit te drukken met behulp van genetische code-uitbreiding en gereconstitueerde nucleosomen in vitro te assembleren.

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Lysine Acetylation Histones Nucleosome Reconstitution Genetic Code Expansion Escherichia Coli Mononucleosome Epigenetic Experiments Biochemical Assays Binding Assays Structure Determination Histone Lysis Buffer Inclusion Bodies Nickel-NTA Purification Acetylated Histone Protein Dialysis Acetic Acid Buffer

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Site specifieke Lysine acetylatie van histonen voor nucleosoom reconstitutie met behulp van genetische code uitbreiding in Escherichia coli

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Summary

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