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Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry

Journal JoVE
Biochimie

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Journal JoVE Biochimie

Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry

Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry

4,323 Views

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07:27 min

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April 09, 2021

DOI:

10.3791/62107-v

DOI:

10.3791/62107-v

•

07:27 min

April 09, 2021

•

9 Views

Lucia M. Q. Liao, Rachel A. V. Gray, Dale D. O. Martin

¹Department of Biology,University of Waterloo

Tags

Alkynyl Fatty Acid Analogs Click Chemistry Fatty Acylated Proteins Fatty Acid Labeling Metabolic Labeling HEK-293 T-cells BSA-bound Fatty Acids Saponification Potassium Hydroxide Fatty Acid-free BSA

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