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The Preparation of Chicken Ex Ovo Embryos and Chorioallantoic Membrane Vessels as In Vivo Model for Contrast-Enhanced Ultrasound Imaging and Microbubble-Mediated Drug Delivery Studies
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
The Preparation of Chicken Ex Ovo Embryos and Chorioallantoic Membrane Vessels as In Vivo Model for Contrast-Enhanced Ultrasound Imaging and Microbubble-Mediated Drug Delivery Studies

The Preparation of Chicken Ex Ovo Embryos and Chorioallantoic Membrane Vessels as In Vivo Model for Contrast-Enhanced Ultrasound Imaging and Microbubble-Mediated Drug Delivery Studies

English

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3,559 Views

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09:53 min

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February 09, 2021

DOI:

10.3791/62076-v

DOI:
10.3791/62076-v

•

09:53 min
February 09, 2021

•

11 Views
, , , ,
1Department of Biomedical Engineering, Thoraxcenter,Erasmus MC University Medical Center Rotterdam, 2Laboratory of Acoustical Wavefield Imaging, Faculty of Applied Sciences,Delft University of Technology

Summary

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This protocol describes three methods on how to obtain and use 5 to 8-day old chicken embryos and their chorioallantoic membrane (CAM) as an in vivo model to study contrast-enhanced ultrasound imaging and microbubble-mediated drug delivery.

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Cite this Article

Meijlink, B., Skachkov, I., van der Steen, A. F. W., de Jong, N., Kooiman, K. The Preparation of Chicken Ex Ovo Embryos and Chorioallantoic Membrane Vessels as In Vivo Model for Contrast-Enhanced Ultrasound Imaging and Microbubble-Mediated Drug Delivery Studies. J. Vis. Exp. (168), e62076, doi:10.3791/62076 (2021).

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