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The Preparation of Chicken Ex Ovo Embryos and Chorioallantoic Membrane Vessels as In Vivo Model for Contrast-Enhanced Ultrasound Imaging and Microbubble-Mediated Drug Delivery Studies
Journal JoVE
Bio-ingénierie
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Journal JoVE Bio-ingénierie
The Preparation of Chicken Ex Ovo Embryos and Chorioallantoic Membrane Vessels as In Vivo Model for Contrast-Enhanced Ultrasound Imaging and Microbubble-Mediated Drug Delivery Studies

The Preparation of Chicken Ex Ovo Embryos and Chorioallantoic Membrane Vessels as In Vivo Model for Contrast-Enhanced Ultrasound Imaging and Microbubble-Mediated Drug Delivery Studies

DOI:
10.3791/62076-v

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09:53 min

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February 09, 2021

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, , , ,
1Department of Biomedical Engineering, Thoraxcenter,Erasmus MC University Medical Center Rotterdam, 2Laboratory of Acoustical Wavefield Imaging, Faculty of Applied Sciences,Delft University of Technology

Chapitres

  • 00:05Introduction
  • 00:43Embryo Preparation
  • 05:24Removing Embryo and CAM from Yolk for Microscopy Imaging
  • 07:25Results: Representative In Vivo Contrast-Enhanced Ultrasound Imaging and Microbubble-Mediated Drug Delivery
  • 09:06Conclusion

Summary

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Traduction automatique

This protocol describes three methods on how to obtain and use 5 to 8-day old chicken embryos and their chorioallantoic membrane (CAM) as an in vivo model to study contrast-enhanced ultrasound imaging and microbubble-mediated drug delivery.

Tags

Chicken EmbryoEx OvoChorioallantoic MembraneContrast-enhanced UltrasoundMicrobubble-mediated Drug DeliveryIn Vivo ModelEgg IncubationEggshell OpeningAlbumin RemovalEmbryo VisualizationYolk Exposure

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