Journal
/
/
Estimering af krystallinsk celluloseindhold i plantebiomasse ved hjælp af Updegraff-metoden
JoVE Journal
Environment
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Environment
Estimation of Crystalline Cellulose Content of Plant Biomass using the Updegraff Method
DOI:

12:34 min

May 15, 2021

, ,

Chapters

  • 00:00Introduction
  • 01:12Phase I: Preparation of Samples
  • 02:02Phase II: Preparation of the Alcohol Insoluble Crude Cell Wall
  • 04:52Phase III: Removal of Hemicellulose and Lignin using Updegraff Reagent
  • 07:10Phase IV: Acid Hydrolysis of Cellulose into Glucose Monomers
  • 07:49Phase V: Anthrone Assay to Estimate the Glucose Concentration of Samples and Glucose Standard
  • 10:04Representative Results & Conclusion

Summary

Automatic Translation

Updegraff-metoden er den mest anvendte metode til celluloseestimatet. Hovedformålet med denne demonstration er at tilvejebringe en detaljeret Updegraff-protokol til vurdering af celluloseindholdet i plantebiomasseprøver.

Related Videos

Read Article