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Estimation of Crystalline Cellulose Content of Plant Biomass using the Updegraff Method
JoVE 杂志
环境科学
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JoVE 杂志 环境科学
Estimation of Crystalline Cellulose Content of Plant Biomass using the Updegraff Method

Estimation of Crystalline Cellulose Content of Plant Biomass using the Updegraff Method

DOI:
10.3791/62031-v

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12:34 min

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May 15, 2021

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, ,
1Department of Plant and Soil Science,Texas Tech University, 2Fiber and Biopolymer Research Institute (FBRI), Department of Plant and Soil Science,Texas Tech University

Chapters

  • 00:00Introduction
  • 01:12Phase I: Preparation of Samples
  • 02:02Phase II: Preparation of the Alcohol Insoluble Crude Cell Wall
  • 04:52Phase III: Removal of Hemicellulose and Lignin using Updegraff Reagent
  • 07:10Phase IV: Acid Hydrolysis of Cellulose into Glucose Monomers
  • 07:49Phase V: Anthrone Assay to Estimate the Glucose Concentration of Samples and Glucose Standard
  • 10:04Representative Results & Conclusion

Summary

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The Updegraff method is the most widely used method for the cellulose estimation. The main purpose of this demonstration is to provide a detailed Updegraff protocol for estimation of cellulose content in plant biomass samples.

Tags

Crystalline CelluloseUpdegraff MethodPlant BiomassCellulose Content EstimationCell Wall ExtractionHemicellulose And Lignin RemovalAcid HydrolysisGlucose MonomersAnthrone Assay

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