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Developmental Biology
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使用免疫荧光检测斑马鱼胚胎心脏PM
2.5
诱发DNA损伤
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Using Immunofluorescence to Detect PM
2.5
-induced DNA Damage in Zebrafish Embryo Hearts
Please note that all translations are automatically generated.
Click here for the English version.
使用免疫荧光检测斑马鱼胚胎心脏PM
2.5
诱发DNA损伤
DOI:
10.3791/62021-v
•
05:58 min
•
February 15, 2021
•
Yujie Huang*
1
,
Yizhou Tao*
1
,
Chang Cai
,
Jin Chen
,
Cheng Ji
,
Stanley Aniagu
,
Yan Jiang
,
Tao Chen
1
School of Public Health
,
Soochow University
,
2
Toxicology, Risk Assessment and Research Division
,
Texas Commission on Environmental Quality
Chapters
00:06
Introduction
00:34
Morphological Observation of Zebrafish Embryos and Cardiac Dissection
01:40
Immunofluorescence Assay
04:29
Results: Cardiac Defects and DNA Damage of Zebrafish Embryos at 72 hpf
05:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议使用免疫荧光检测检测斑马鱼胚胎解剖心脏中
PM2.5
诱发的DNA损伤。
Tags
Immunofluorescence
PM2.5
DNA Damage
Zebrafish Embryo
Heart
8-hydroxydeoxyguanosine
Gamma-H2AX
Antibody
Fixation
Immunostaining
Microscopy
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