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10:28 min
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April 03, 2021
DOI:
1Center for Gene Therapy,The Research Institute at Nationwide Children’s Hospital, 2Center for Cardiovascular Research,The Research Institute at Nationwide Children’s Hospital, 3The Heart Center,Nationwide Children’s Hospital, 4Division of Genetic and Genomic Medicine,Nationwide Children’s Hospital, 5Department of Human Genetics,The University of Utah School of Medicine, 6Department of Pediatrics,The Ohio State University
Summary
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Este protocolo descreve a conversão de fibroblastos de pele em míobios e sua diferenciação em miotubes. As linhas celulares são derivadas de pacientes com distúrbios neuromusculares e podem ser usadas para investigar mecanismos patológicos e testar estratégias terapêuticas.
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Cite this Article
Almeida, C. F., Frair, E. C., Huang, N., Neinast, R., McBride, K. L., Weiss, R. B., Flanigan, K. M., Wein, N. Direct Reprogramming of Human Fibroblasts into Myoblasts to Investigate Therapies for Neuromuscular Disorders. J. Vis. Exp. (170), e61991, doi:10.3791/61991 (2021).
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