A subscription to JoVE is required to view this content. Sign in or start your free trial.
5,816 Views
•
10:28 min
•
April 03, 2021
DOI:
1Center for Gene Therapy,The Research Institute at Nationwide Children’s Hospital, 2Center for Cardiovascular Research,The Research Institute at Nationwide Children’s Hospital, 3The Heart Center,Nationwide Children’s Hospital, 4Division of Genetic and Genomic Medicine,Nationwide Children’s Hospital, 5Department of Human Genetics,The University of Utah School of Medicine, 6Department of Pediatrics,The Ohio State University
Summary
Automatically generated
Ce protocole décrit la conversion des fibroblastes cutanés en myoblastes et leur différenciation en myotubes. Les lignées cellulaires sont dérivées de patients atteints de troubles neuromusculaires et peuvent être utilisées pour étudier les mécanismes pathologiques et tester des stratégies thérapeutiques.
Read Article
Cite this Article
Almeida, C. F., Frair, E. C., Huang, N., Neinast, R., McBride, K. L., Weiss, R. B., Flanigan, K. M., Wein, N. Direct Reprogramming of Human Fibroblasts into Myoblasts to Investigate Therapies for Neuromuscular Disorders. J. Vis. Exp. (170), e61991, doi:10.3791/61991 (2021).
Copy