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Cristalização e Determinação Estrutural de uma Enzima:Complexo de Substrato por Cristalografia Serial em um Chip Microfluido Versátil
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip

Cristalização e Determinação Estrutural de uma Enzima:Complexo de Substrato por Cristalografia Serial em um Chip Microfluido Versátil

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7,002 Views

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10:45 min

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March 20, 2021

DOI:

10.3791/61972-v

DOI:
10.3791/61972-v

•

10:45 min
March 20, 2021

•

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1Université de Strasbourg, Architecture et Réactivité de l’ARN, UPR 9002, CNRS,Institut de Biologie Moléculaire et Cellulaire, 2Biochemistry and Molecular Biology, Institute for Biochemistry,Leipzig University, 3Paul Scherrer Institute, Swiss Light Source, 4European XFEL GmbH

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de Wijn, R., Rollet, K., Olieric, V., Hennig, O., Thome, N., Noûs, C., Paulus, C., Lorber, B., Betat, H., Mörl, M., Sauter, C. Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip. J. Vis. Exp. (169), e61972, doi:10.3791/61972 (2021).

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