Journal
/
Biochemistry
/
酵素の結晶化と構造決定:多用途マイクロ流体チップにおける連続結晶学による基質複合体
JoVE Journal
Biochemistry
Author Produced
This content is Free Access.
JoVE Journal Biochemistry
Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip
Please note that all translations are automatically generated. Click here for the English version.

酵素の結晶化と構造決定:多用途マイクロ流体チップにおける連続結晶学による基質複合体

日本語

Automatically Generated

7,792 Views

•

10:45 min

•

March 20, 2021

DOI:

10.3791/61972-v

DOI:
10.3791/61972-v

•

10:45 min
March 20, 2021

•

22 Views
, , , , , , , , , ,
1Université de Strasbourg, Architecture et Réactivité de l’ARN, UPR 9002, CNRS,Institut de Biologie Moléculaire et Cellulaire, 2Biochemistry and Molecular Biology, Institute for Biochemistry,Leipzig University, 3Paul Scherrer Institute, Swiss Light Source, 4European XFEL GmbH

Summary

Automatically generated

多目的なマイクロ流体デバイスは、カウンター拡散法を用いた酵素の結晶化、浸漬による結晶中の基質の導入、および室温でチップ内部の結晶の逐次分析による酵素:基質複合体の3D構造決定を可能にする記載されている。

Explore More Videos

CrystallizationStructural DeterminationEnzyme-substrate ComplexSerial CrystallographyMicrofluidic ChipCounter-diffusionIn Situ X-ray DiffractionBiomoleculeCrystal GrowthRoom Temperature Analysis

Read Article

Cite this Article

de Wijn, R., Rollet, K., Olieric, V., Hennig, O., Thome, N., Noûs, C., Paulus, C., Lorber, B., Betat, H., Mörl, M., Sauter, C. Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip. J. Vis. Exp. (169), e61972, doi:10.3791/61972 (2021).

Download .ris file

Copy

Share Video

.

Copy