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A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells

A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells

English

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4,209 Views

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07:14 min

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May 17, 2021

DOI:

10.3791/61938-v

DOI:
10.3791/61938-v

•

07:14 min
May 17, 2021

•

12 Views
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1Department of Stem Cell Biology, Research Institute,National Center for Global Health and Medicine

Summary

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This protocol enables the maintenance of quiescent human hematopoietic stem cells in vitro by mimicking the bone marrow microenvironment using abundant fatty acids, cholesterol, lower concentrations of cytokines, and hypoxia.

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Cite this Article

Kobayashi, H., Takubo, K. A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells. J. Vis. Exp. (171), e61938, doi:10.3791/61938 (2021).

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