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A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
JoVE Journal
Bioingegneria
This content is Free Access.
JoVE Journal Bioingegneria
A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells

A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells

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07:14 min

May 17, 2021

DOI:

07:14 min
May 17, 2021

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Summary

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This protocol enables the maintenance of quiescent human hematopoietic stem cells in vitro by mimicking the bone marrow microenvironment using abundant fatty acids, cholesterol, lower concentrations of cytokines, and hypoxia.

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