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A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
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May 17, 2021
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This protocol enables the maintenance of quiescent human hematopoietic stem cells in vitro by mimicking the bone marrow microenvironment using abundant fatty acids, cholesterol, lower concentrations of cytokines, and hypoxia.
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Kobayashi, H., Takubo, K. A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells. J. Vis. Exp. (171), e61938, doi:10.3791/61938 (2021).
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