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High-Throughput Metabolic Profiling for Model Refinements of Microalgae
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JoVE Journal Biology
High-Throughput Metabolic Profiling for Model Refinements of Microalgae

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

DOI:
10.3791/61913-v

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11:07 min

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December 04, 2021

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, , , , , , , ,
1Center for Genomics and Systems Biology (CGSB),New York University Abu Dhabi Research Institute, 2Department of Biology,United Arab Emirates University, 3Laboratory of Algal, Systems, and Synthetic Biology (LASSB), Division of Science and Math,New York University Abu Dhabi, 4Department of Marine Science, Ocean College,Zhejiang University

Chapters

  • 00:07Introduction
  • 01:13Phenotype Microarray Experiments
  • 03:58Data Extraction and Analysis
  • 05:06Identification of Reactions and Genes Associated with New Metabolites
  • 06:41Model Refinement and Evaluation
  • 08:38Results
  • 10:25Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol demonstrates the use of a phenotype microarray (PM) technology platform to define metabolic requirements of Chlamydomonas reinhardtii, a green microalga, and refine an existing metabolic network model.

Tags

Keywords: Metabolic ProfilingMicroalgaePhenotype Micro-arrayHigh-throughputChlamydomonas ReinhardtiiMetabolic Network ModelsTetrazolium-based Redox DyeCarbon SourcesNitrogen SourcesPhosphorusSulfurPeptide Nitrogen SourcesHeterotrophic RespirationMicroplate Reader

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