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High-Throughput Metabolic Profiling for Model Refinements of Microalgae
 

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

Article DOI: 10.3791/61913-v • 11:07 min • December 4th, 2021
December 4th, 2021 •
1,2, 1, 3, 3, 3, 3,4, 1, 1, 1,3
1Center for Genomics and Systems Biology (CGSB), New York University Abu Dhabi Research Institute, 2Department of Biology, United Arab Emirates University, 3Laboratory of Algal, Systems, and Synthetic Biology (LASSB), Division of Science and Math, New York University Abu Dhabi, 4Department of Marine Science, Ocean College, Zhejiang University

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This protocol demonstrates the use of a phenotype microarray (PM) technology platform to define metabolic requirements of Chlamydomonas reinhardtii, a green microalga, and refine an existing metabolic network model.

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Keywords: Metabolic Profiling Microalgae Phenotype Micro-array High-throughput Chlamydomonas Reinhardtii Metabolic Network Models Tetrazolium-based Redox Dye Carbon Sources Nitrogen Sources Phosphorus Sulfur Peptide Nitrogen Sources Heterotrophic Respiration Microplate Reader
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