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Microinjection d’oeufs et accouplement efficace pour l’édition du génome dans le Firebrat Thermobia domestica
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Developmental Biology
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JoVE Journal Developmental Biology
Egg Microinjection and Efficient Mating for Genome Editing in the Firebrat Thermobia domestica
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Microinjection d’oeufs et accouplement efficace pour l’édition du génome dans le Firebrat Thermobia domestica

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4,187 Views

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06:08 min

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October 20, 2020

DOI:

10.3791/61885-v

DOI:
10.3791/61885-v

•

06:08 min
October 20, 2020

•

3 Views
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1Department of Applied Biosciences, Graduate School of Agriculture,Kyoto University

Summary

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Nous fournissons un protocole détaillé pour l’élevage, la microinjection des œufs et pour l’accouplement efficace du firebrat Thermobia domestica pour générer et maintenir des souches mutantes après l’édition du génome.

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Thermobia DomesticaFirebratEgg MicroinjectionGenome EditingInsect ModelCulture MaintenanceRNAiTransgenesisLaboratory Model

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Ohde, T., Minemura, T., Hirose, E., Daimon, T. Egg Microinjection and Efficient Mating for Genome Editing in the Firebrat Thermobia domestica. J. Vis. Exp. (164), e61885, doi:10.3791/61885 (2020).

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