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Egg Microinjection and Efficient Mating for Genome Editing in the Firebrat Thermobia domestica
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Egg Microinjection and Efficient Mating for Genome Editing in the Firebrat Thermobia domestica
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October 20, 2020
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We provide a detailed protocol for rearing, microinjection of eggs and for efficient mating of the firebrat Thermobia domestica to generate and maintain mutant strains after genome editing.
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Ohde, T., Minemura, T., Hirose, E., Daimon, T. Egg Microinjection and Efficient Mating for Genome Editing in the Firebrat Thermobia domestica. J. Vis. Exp. (164), e61885, doi:10.3791/61885 (2020).
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