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PCR変異生成、クローニング、発現、高速タンパク質精製プロトコルおよびトリプトファン合成酵素の野生型および変異型の結晶化
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Biochemistry
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JoVE Journal Biochemistry
PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
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PCR変異生成、クローニング、発現、高速タンパク質精製プロトコルおよびトリプトファン合成酵素の野生型および変異型の結晶化

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09:31 min

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September 26, 2020

DOI:

10.3791/61839-v

DOI:
10.3791/61839-v

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09:31 min
September 26, 2020

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1Department of Chemistry,University of California-Riverside, 2Department of Biochemistry,University of California-Riverside

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PCR MutagenesisCloningExpressionProtein PurificationTryptophan SynthaseSalmonella TyphimuriumAmmonium Sulfate FractionationSize Exclusion ChromatographySDS-PAGEProtein ConcentrationCrystallization

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Hilario, E., Fan, L., Mueller, L. J., Dunn, M. F. PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase. J. Vis. Exp. (163), e61839, doi:10.3791/61839 (2020).

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