PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase

Eduardo Hilario; Li Fan; Leonard  J. Mueller; Michael  F. Dunn

doi:10.3791/61839

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PCR-Mutagenese, Klonierung, Expression, schnelle Proteinreinigungsprotokolle und Kristallisation des Wildtyps und der mutierten Formen der Tryptophan-Synthase

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase