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October 07, 2020
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1Institute of Biomedicine, Integrative Physiology and Pharmacology Unit,University of Turku, Turku, Finland, 2Department of Pediatrics,Turku University Hospital, Turku, Finland, 3Centre for Reproductive Health,Hudson Institute of Medical Research, Melbourne, VIC 3168, Australia, 4Australian Regenerative Medicine Institute,Monash University, Melbourne, VIC 3800, Australia
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本プロトコルは、半日本円上皮周期の特定段階を表す成体マウス半細管の断面微小切除法、およびその中の細胞タイプ、および後にスカッシュ製剤および無傷の管状セグメントの免疫染色を記述する。
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Mäkelä, J., Cisneros-Montalvo, S., Lehtiniemi, T., Olotu, O., La, H. M., Toppari, J., Hobbs, R. M., Parvinen, M., Kotaja, N. Transillumination-Assisted Dissection of Specific Stages of the Mouse Seminiferous Epithelial Cycle for Downstream Immunostaining Analyses. J. Vis. Exp. (164), e61800, doi:10.3791/61800 (2020).
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