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Transillumination-Assisted Dissection of Specific Stages of the Mouse Seminiferous Epithelial Cycle for Downstream Immunostaining Analyses
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October 07, 2020
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1Institute of Biomedicine, Integrative Physiology and Pharmacology Unit,University of Turku, Turku, Finland, 2Department of Pediatrics,Turku University Hospital, Turku, Finland, 3Centre for Reproductive Health,Hudson Institute of Medical Research, Melbourne, VIC 3168, Australia, 4Australian Regenerative Medicine Institute,Monash University, Melbourne, VIC 3800, Australia
Summary
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Dieses Protokoll beschreibt die transilluminationsunterstützte Mikrodissektion von Segmenten von erwachsenen Mausseminiferous Tubuli, die bestimmte Stadien des seminiferen Epithelzyklus darstellen, und Zelltypen darin und anschließende Immunostainierung von Squashpräparaten und intakten Tubulisegmenten.
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Mäkelä, J., Cisneros-Montalvo, S., Lehtiniemi, T., Olotu, O., La, H. M., Toppari, J., Hobbs, R. M., Parvinen, M., Kotaja, N. Transillumination-Assisted Dissection of Specific Stages of the Mouse Seminiferous Epithelial Cycle for Downstream Immunostaining Analyses. J. Vis. Exp. (164), e61800, doi:10.3791/61800 (2020).
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