/
/
A Protocol for Immunohistochemistry and RNA In-situ Distribution within Early Drosophila Embryo
A subscription to JoVE is required to view this content. Sign in or start your free trial.
3,414 Views
•
14:34 min
•
May 06, 2022
DOI:
1National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China/CGDB,Shaanxi Normal University College of Life Sciences, 2Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Shaanxi Key Laboratory of Brain disorders, Institute of Basic & Translational Medicine,Xi’an Medical University, 3Ohio State University College of Medicine, 4Hematology-Oncology Division, Department of Medicine, Beth Israel Deaconess Medical Center,Harvard Medical School, 5University of Pennsylvania ULAR, 6University of Maryland School of Medicine, 7Columbia University Medical Center
Summary
Automatically generated
Here, we describe a protocol for detection and localization of Drosophila embryo protein and RNA from collection to pre-embedding and embedding, immunostaining, and mRNA in situ hybridization.
Read Article
Cite this Article
Zhang, W., Lei, X., Zhou, X., He, B., Xiao, L., Yue, H., Wang, S., Sun, Y., Wu, Y., Wang, L., Ghartey-Kwansah, G., Jones, O. D., Bryant, J. L., Xu, M., Ma, J., Xu, X. A Protocol for Immunohistochemistry and RNA In-situ Distribution within Early Drosophila Embryo. J. Vis. Exp. (183), e61776, doi:10.3791/61776 (2022).
Copy