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A Protocol for Immunohistochemistry and RNA In-situ Distribution within Early Drosophila Embryo
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14:34 min
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May 06, 2022
DOI:
1National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China/CGDB,Shaanxi Normal University College of Life Sciences, 2Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Shaanxi Key Laboratory of Brain disorders, Institute of Basic & Translational Medicine,Xi’an Medical University, 3Ohio State University College of Medicine, 4Hematology-Oncology Division, Department of Medicine, Beth Israel Deaconess Medical Center,Harvard Medical School, 5University of Pennsylvania ULAR, 6University of Maryland School of Medicine, 7Columbia University Medical Center
Summary
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Here, we describe a protocol for detection and localization of Drosophila embryo protein and RNA from collection to pre-embedding and embedding, immunostaining, and mRNA in situ hybridization.
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Zhang, W., Lei, X., Zhou, X., He, B., Xiao, L., Yue, H., Wang, S., Sun, Y., Wu, Y., Wang, L., Ghartey-Kwansah, G., Jones, O. D., Bryant, J. L., Xu, M., Ma, J., Xu, X. A Protocol for Immunohistochemistry and RNA In-situ Distribution within Early Drosophila Embryo. J. Vis. Exp. (183), e61776, doi:10.3791/61776 (2022).
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