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Drosophila Embryo Preparation and Microinjection for Live Cell Microscopy Performed using an Automated High Content Analyzer
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Drosophila Embryo Preparation and Microinjection for Live Cell Microscopy Performed using an Automated High Content Analyzer

Drosophila Embryo Preparation and Microinjection for Live Cell Microscopy Performed using an Automated High Content Analyzer

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7,999 Views

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05:52 min

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January 19, 2021

DOI:

10.3791/61589-v

DOI:
10.3791/61589-v

•

05:52 min
January 19, 2021

•

8 Views
, ,
1Department of Biology,San Francisco State University, 2Department of Biochemistry & Biophysics,UCSF Mission Bay

Summary

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Presented here is a protocol to microinject and simultaneously image multiple Drosophila embryos during embryonic development using a plate-based, high content imager.

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Cite this Article

Diaz, U., Marshall, W., Riggs, B. Drosophila Embryo Preparation and Microinjection for Live Cell Microscopy Performed using an Automated High Content Analyzer. J. Vis. Exp. (167), e61589, doi:10.3791/61589 (2021).

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