Drosophila Embryo Preparation and Microinjection for Live Cell Microscopy Performed using an Automated High Content Analyzer

Ulises Diaz; Wallace Marshall; Blake Riggs

doi:10.3791/61589

/

Drosophila Embryo Preparation and Microinjection for Live Cell Microscopy Performed using an Automated High Content Analyzer

JoVE Journal
Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Drosophila Embryo Preparation and Microinjection for Live Cell Microscopy Performed using an Automated High Content Analyzer

8,052 Views

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05:52 min

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January 19, 2021

DOI:

10.3791/61589-v

DOI:

10.3791/61589-v

•

05:52 min

January 19, 2021

•

8 Views

Ulises Diaz^1,2, Wallace Marshall², Blake Riggs¹

¹Department of Biology,San Francisco State University, ²Department of Biochemistry & Biophysics,UCSF Mission Bay

Summary

Presented here is a protocol to microinject and simultaneously image multiple Drosophila embryos during embryonic development using a plate-based, high content imager.

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Drosophila Embryo Preparation and Microinjection for Live Cell Microscopy Performed using an Automated High Content Analyzer

Summary

Automatically generated

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