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Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish
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JoVE Journal Medicine
Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish

Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish

English

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5,077 Views

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13:25 min

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May 15, 2021

DOI:

10.3791/61575-v

DOI:
10.3791/61575-v

•

13:25 min
May 15, 2021

•

7 Views
, , , , , ,
1Department of Immunology,University of São Paulo, 2Department of Medicine, Nephrology Division,Federal University of São Paulo

Summary

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This protocol describes the procedures to induce Acute Kidney Injury (AKI) in adult zebrafish using cisplatin as a nephrotoxic agent. We detailed the steps to evaluate the reproducibility of the technique and two techniques to analyze inflammation and cell death in the renal tissue, flow cytometry and TUNEL, respectively.

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Acute Kidney InjuryCisplatinZebrafishNephrotoxicityInflammationCell DeathFlow CytometryTUNEL AssayChemotherapyRenal ProtectionRegeneration

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Cite this Article

Morales Fénero, C., Padovani, B. N., do Amaral, M. A., de Barros, G. J. B., de Oliveira, I. K. X., Hiyane, M. I., Camâra, N. O. S. Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish. J. Vis. Exp. (171), e61575, doi:10.3791/61575 (2021).

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