Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish

Camila Morales F&#233;nero; Barbara  Nunes Padovani; Mariana  Abrantes do Amaral; Guilherme Jos&#233;  Bottura de Barros; Izabella Karina  Xavier de Oliveira; Meire  Ioshie Hiyane; Niels Olsen  Saraiva Cam&#226;ra

doi:10.3791/61575

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Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish

JoVE Journal
Medicina
Author Produced

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JoVE Journal Medicina

Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish

5,078 Views

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13:25 min

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May 15, 2021

DOI:

10.3791/61575-v

DOI:

10.3791/61575-v

•

13:25 min

May 15, 2021

•

7 Views

Camila Morales Fénero*¹, Barbara Nunes Padovani*¹, Mariana Abrantes do Amaral*^1,2, Guilherme José Bottura de Barros¹, Izabella Karina Xavier de Oliveira¹, Meire Ioshie Hiyane¹, Niels Olsen Saraiva Camâra^1,2

¹Department of Immunology,University of São Paulo, ²Department of Medicine, Nephrology Division,Federal University of São Paulo

Summary

This protocol describes the procedures to induce Acute Kidney Injury (AKI) in adult zebrafish using cisplatin as a nephrotoxic agent. We detailed the steps to evaluate the reproducibility of the technique and two techniques to analyze inflammation and cell death in the renal tissue, flow cytometry and TUNEL, respectively.

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Acute Kidney Injury Cisplatin Zebrafish Nephrotoxicity Inflammation Cell Death Flow Cytometry TUNEL Assay Chemotherapy Renal Protection Regeneration

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Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish

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