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Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis
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JoVE Journal Biyokimya
Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

DOI:
10.3791/61489-v

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05:47 min

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June 25, 2020

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, , ,
1Department of Plant Biology,Carnegie Institute for Science, 2Farmasötik Bilimler Enstitüsü,Kyoto University, 3Department of Biochemistry,Purdue Üniversitesi, 4Kimya Bölümü,Purdue Üniversitesi, 5Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences

Bölümler

  • 00:04Introduction
  • 00:33Immobilized Metal Ion Affinity Chromatography (IMAC) and High pH Reversed-Phase (Hp-RP) Stage Tip Preparation
  • 01:10IMAC Stage Tip Phosphopeptide Enrichment
  • 02:44Hp-RP C18 Stage Tip Phosphopeptide Fractionation
  • 03:37Results: Representative Osmotic Stress Signaling in Arabidopsis
  • 05:05Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Presented here is a phosphoproteomic approach, namely stop and go extraction tip based phosphoproteomic, which provides high-throughput and deep coverage of Arabidopsis phosphoproteome. This approach delineates the overview of osmotic stress signaling in Arabidopsis.

Etiketler

PhosphoproteomicsOsmotic StressArabidopsisIMACPhosphopeptide EnrichmentHigh PH Reverse Phase Fractionation

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