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Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification
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Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

DOI:
10.3791/61478-v

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07:25 min

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June 25, 2020

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, , , , ,
1Department of Plant Pathology, Coastal Plain Experiment Station,University of Georgia, 2University of Georgia Cooperative Extension, 3Department of Plant Pathology, Tropical Research & Education Center,University of Florida, 4Department of Agricultural and Environmental Sciences, Otis L. Floyd Nursery Research Center,Tennessee State University

Capitoli

  • 00:04Introduction
  • 00:48Pump and Filter Setup
  • 01:16Water Sampling and Filtering
  • 02:22Magnetic Bead-Based DNA Extraction
  • 03:58Loop-Mediated Isothermal Amplification (LAMP) Assay
  • 05:07Results: Representative Phytophthora capsici Detection
  • 06:53Conclusion

Summary

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We developed a method to detect Phytophthora capsici zoospores in water sources using a filter paper DNA extraction method coupled with a loop-mediated isothermal amplification (LAMP) assay that can be analyzed in the field or in the lab.

Tags

Phytophthora CapsiciIrrigation WaterLoop-mediated Isothermal AmplificationPathogen DetectionDNA ExtractionFilter PaperMagnetic BeadsIrrigation Water ContaminationWaterborne Pathogen

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