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Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin
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11:17 min
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March 10, 2021
DOI:
1School of Laboratory Medicine and Life Sciences,Wenzhou Medical University, 2NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention, 3Faculty of Health Sciences,University of Macau, 4CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology,Chinese Academy of Sciences, 5Savaid Medical School,University of Chinese Academy of Sciences
Summary
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The protocol describes experimental methods to obtain stable major histocompatibility complex (MHC) class I through potential β2-microglobulin (β2m) substitutions from different species. The structural comparison of MHC I stabilized by homologous and heterologous β2m were investigated.
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Zhang, D., Liu, K., Lu, D., Wang, P., Zhang, Q., Liu, P., Zhao, Y., Chai, Y., Lyu, J., Qi, J., Liu, W. J. Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin. J. Vis. Exp. (169), e61462, doi:10.3791/61462 (2021).
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