The protocol describes experimental methods to obtain stable major histocompatibility complex (MHC) class I through potential β2-microglobulin (β2m) substitutions from different species. The structural comparison of MHC I stabilized by homologous and heterologous β2m were investigated.
Zhang, D., Liu, K., Lu, D., Wang, P., Zhang, Q., Liu, P., Zhao, Y., Chai, Y., Lyu, J., Qi, J., Liu, W. J. Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin. J. Vis. Exp. (169), e61462, doi:10.3791/61462 (2021).