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Super-Resolution Imaging zur Untersuchung der Kolokalisierung von Proteinen und synaptischen Markern in primärer Neuronen
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Neuroscience
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JoVE Journal Neuroscience
Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons
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Super-Resolution Imaging zur Untersuchung der Kolokalisierung von Proteinen und synaptischen Markern in primärer Neuronen

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5,624 Views

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14:02 min

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October 31, 2020

DOI:

10.3791/61434-v

DOI:
10.3791/61434-v

•

14:02 min
October 31, 2020

•

3 Views
, , , , , , , ,
1Department of Molecular Biochemistry and Pharmacology,Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 2Department of Neuroscience,Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 3Dulbecco Telethon Institute,Istituto di Ricerche Farmacologiche Mario Negri IRCCS

Summary

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Dieses Protokoll zeigt, wie man eine hochauflösende Mikroskopie einsetzt, um die Proteinkolokalisierung in primären neuronalen Kulturen zu untersuchen.

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Russo, L., Natale, C., Conz, A., Kelk, J., Restelli, E., Chiesa, R., Salmona, M., Fioriti, L., Colnaghi, L. Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons. J. Vis. Exp. (164), e61434, doi:10.3791/61434 (2020).

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