Journal
/
Neurosciences
/
Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons
Journal JoVE
Neurosciences
Author Produced
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Neurosciences
Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

5,623 Views

•

14:02 min

•

October 31, 2020

DOI:

10.3791/61434-v

DOI:
10.3791/61434-v

•

14:02 min
October 31, 2020

•

3 Views
, , , , , , , ,
1Department of Molecular Biochemistry and Pharmacology,Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 2Department of Neuroscience,Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 3Dulbecco Telethon Institute,Istituto di Ricerche Farmacologiche Mario Negri IRCCS

Summary

Automatically generated

This protocol shows how to employ super-resolution microscopy to study protein co-localization in primary neuronal cultures.

Explore More Videos

Super-resolution ImagingCo-localizationPrimary NeuronsSynaptic MarkersStructured Illumination Microscopy (SIM)Neurodegenerative DisordersHippocampal NeuronsFixationImmunostainingMounting SolutionCoverglass

Read Article

Cite this Article

Russo, L., Natale, C., Conz, A., Kelk, J., Restelli, E., Chiesa, R., Salmona, M., Fioriti, L., Colnaghi, L. Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons. J. Vis. Exp. (164), e61434, doi:10.3791/61434 (2020).

Download .ris file

Copy

Share Video

.

Copy