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Isolamento e Cultura de Chick Ciliary Ganglion Neurons
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Neuroscience
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JoVE Journal Neuroscience
Isolation and Culture of Chick Ciliary Ganglion Neurons

Isolamento e Cultura de Chick Ciliary Ganglion Neurons

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5,593 Views

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14:36 min

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August 08, 2020

DOI:

10.3791/61431-v

DOI:
10.3791/61431-v

•

14:36 min
August 08, 2020

•

1 Views
, , , ,
1Institute of Biomedicine, Department of Medical Sciences – iBiMED,University of Aveiro, 2CNC – Center for Neuroscience and Cell Biology,University of Coimbra

Summary

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As gânglios ciliares filhotes (CG) fazem parte do sistema nervoso parassimpático. Culturas neuronais de neurônios CG foram mostrados como modelos celulares eficazes no estudo das interações musculares nervosas. Descrevemos um protocolo detalhado para a dissecação, dissociação e cultura in vitro de neurônios CG de embriões de filhotes.

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Costa, F. J., Dias, M. S., Costa, R. O., Pedro, J. R., Almeida, R. D. Isolation and Culture of Chick Ciliary Ganglion Neurons. J. Vis. Exp. (162), e61431, doi:10.3791/61431 (2020).

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