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Isolation and Culture of Chick Ciliary Ganglion Neurons
Bölümler
- 00:00Introduction
- 01:41Preparation of Coverslips
- 08:00Dissociation and Culture of Chick Ciliary Ganglion Neurons
- 11:42Representative Results
- 12:42Discussion and Conclusion
Chick ciliary ganglia (CG) are part of the parasympathetic nervous system. Neuronal cultures of chick CG neurons were shown to be effective cell models in the study of nerve muscle interactions. We describe a detailed protocol for the dissection, dissociation and in vitro culture of CG neurons from chick embryos.
