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In vitro neuromuskulær junction induceret fra humane inducerede pluripotente stamceller
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells

In vitro neuromuskulær junction induceret fra humane inducerede pluripotente stamceller

DOI:
10.3791/61396-v

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06:01 min

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December 03, 2020

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, , ,
1Department of Clinical Application, Center for iPS Cell Research and Application (CiRA),Kyoto University, 2Department of Pediatrics,Kyoto Prefectural University of Medicine, 3Graduate Institute of Clinical Medicine,Taipei Medical University

Chapters

  • 00:04Introduction
  • 00:59Induced Pluripotent Stem Cell (iPSC) Culture
  • 02:00iPSC Differentiation
  • 03:04Neuromuscular Junction (NMJ) Muscle Contraction and Curare Treatment
  • 03:47Results: Representative NMJ Phenotypic, Morphological, and Functional Characterization
  • 05:20Conclusion

Summary

Automatic Translation

Automatic Translation

Her leverer vi en protokol til at generere in vitro NMJs fra humane inducerede pluripotente stamceller (iPSCs). Denne metode kan fremkalde NMJs med modne morfologi og funktion i 1 måned i en enkelt brønd. De resulterende NMJs kunne potentielt bruges til at modellere relaterede sygdomme, til at studere patologiske mekanismer eller til at screene lægemiddelforbindelser til behandling.

Tags

Human Induced Pluripotent Stem CellsNeuromuscular JunctionMotor NeuronsSkeletal MuscleSchwann CellsSpinal Muscular AtrophyCell DifferentiationCell CultureCell DetachmentExtracellular MatrixDoxycyclineDifferentiation MediumMyogenic Differentiation MediumNeuromuscular Junction Medium

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