Here, we present a simple approach using specific culture media that allows the establishment of neuron- and astrocyte-enriched cultures, or neuron-glia cultures from the embryonic cortex, with high yield and reproducibility.
Gava-Junior, G., Roque, C., Mendes-Oliveira, J., Bernardino, A. C., Serrenho, I., Pires, J. P., Baltazar, G. A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia. J. Vis. Exp. (165), e61388, doi:10.3791/61388 (2020).