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Digestão de olhos inteiros de rato para análise citométrica de fluxo multi-parameter de fagocitos mononucleares
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Immunology and Infection
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JoVE Journal Immunology and Infection
Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes

Digestão de olhos inteiros de rato para análise citométrica de fluxo multi-parameter de fagocitos mononucleares

português

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4,704 Views

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09:58 min

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June 17, 2020

DOI:

10.3791/61348-v

DOI:
10.3791/61348-v

•

09:58 min
June 17, 2020

•

2 Views
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1Department of Ophthalmology, Feinberg School of Medicine,Northwestern University, 2Department of Medicine, Division of Rheumatology, Feinberg School of Medicine,Northwestern University

Summary

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Este protocolo fornece um método para digerir olhos inteiros em uma única suspensão celular com o propósito de análise citométrica de fluxo de vários parâmetros, a fim de identificar populações fagocíticas mononucleares específicas, incluindo monócitos, microglia, macrófagos e células dendríticas.

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Cite this Article

Droho, S., Cuda, C. M., Lavine, J. A. Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes. J. Vis. Exp. (160), e61348, doi:10.3791/61348 (2020).

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