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Vertering van hele muisogen voor multiparameterstroomcytometrische analyse van mononucleaire fagocyten
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Immunology and Infection
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JoVE Journal Immunology and Infection
Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes
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Vertering van hele muisogen voor multiparameterstroomcytometrische analyse van mononucleaire fagocyten

Nederlands

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4,704 Views

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09:58 min

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June 17, 2020

DOI:

10.3791/61348-v

DOI:
10.3791/61348-v

•

09:58 min
June 17, 2020

•

2 Views
, ,
1Department of Ophthalmology, Feinberg School of Medicine,Northwestern University, 2Department of Medicine, Division of Rheumatology, Feinberg School of Medicine,Northwestern University

Summary

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Dit protocol biedt een methode om hele ogen te verteren tot een suspensie met één cel met het oog op cytometrische analyse van de multiparameterstroom om specifieke oculaire mononucleaire fagocytische populaties te identificeren, waaronder monocyten, microglia, macrofagen en dendritische cellen.

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Cite this Article

Droho, S., Cuda, C. M., Lavine, J. A. Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes. J. Vis. Exp. (160), e61348, doi:10.3791/61348 (2020).

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