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Optogenetic Activation of Afferent Pathways in Brain Slices and Modulation of Responses by Volatile Anesthetics
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Neuroscience
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JoVE Journal Neuroscience
Optogenetic Activation of Afferent Pathways in Brain Slices and Modulation of Responses by Volatile Anesthetics

Optogenetic Activation of Afferent Pathways in Brain Slices and Modulation of Responses by Volatile Anesthetics

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2,051 Views

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08:16 min

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July 23, 2020

DOI:

10.3791/61333-v

DOI:
10.3791/61333-v

•

08:16 min
July 23, 2020

•

7 Views
, , ,
1Department of Anesthesiology,University of Wisconsin, 2Department of Anesthesiology, Rambam Health Care Campus, the Ruth and Bruce Rappaport Faculty of Medicine,Technion – Israel Institute of Technology

Summary

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Ex vivo brain slices can be used to study the effects of volatile anesthetics on evoked responses to afferent inputs. Optogenetics are employed to independently activate thalamocortical and corticocortical afferents to non-primary neocortex, and synaptic and network responses are modulated with isoflurane.

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OptogeneticsAfferent PathwaysBrain SlicesVolatile AnestheticsSynapse SpecificityCell Type SpecificityPopulation ActivitySlicing ACSFCoronal Brain SlicesIsofluraneExperimental ACSFMulti-channel Probe

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Murphy, C. A., Raz, A., Grady, S. M., Banks, M. I. Optogenetic Activation of Afferent Pathways in Brain Slices and Modulation of Responses by Volatile Anesthetics. J. Vis. Exp. (161), e61333, doi:10.3791/61333 (2020).

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