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Optogenetic Activation of Afferent Pathways in Brain Slices and Modulation of Responses by Volatile Anesthetics
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JoVE Journal 神経科学
Optogenetic Activation of Afferent Pathways in Brain Slices and Modulation of Responses by Volatile Anesthetics

Optogenetic Activation of Afferent Pathways in Brain Slices and Modulation of Responses by Volatile Anesthetics

DOI:
10.3791/61333-v

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08:16 min

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July 23, 2020

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, , ,
1Department of Anesthesiology,University of Wisconsin, 2Department of Anesthesiology, Rambam Health Care Campus, the Ruth and Bruce Rappaport Faculty of Medicine,Technion – Israel Institute of Technology

章

  • 00:04Introduction
  • 00:45Preparation of Acute Brain Slices
  • 02:47Placing Multi-Channel Probe in Ex Vivo Brain Tissue Slice
  • 04:38Patch Clamping Targeted Neurons and Obtaining Whole-Cell Configuration
  • 05:55Results: Intracellular and Multi-Channel Extracellular Recordings in Cortical Slice
  • 07:19Conclusion

概要

自動翻訳

自動翻訳

Ex vivo brain slices can be used to study the effects of volatile anesthetics on evoked responses to afferent inputs. Optogenetics are employed to independently activate thalamocortical and corticocortical afferents to non-primary neocortex, and synaptic and network responses are modulated with isoflurane.

タグ

OptogeneticsAfferent PathwaysBrain SlicesVolatile AnestheticsSynapse SpecificityCell Type SpecificityPopulation ActivitySlicing ACSFCoronal Brain SlicesIsofluraneExperimental ACSFMulti-channel Probe

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