Oikopleura dioica is a tunicate model organism in various fields of biology. We describe sampling methods, species identification, culturing setup, and culturing protocols for the animals and algal feed. We highlight key factors that helped strengthen the culture system and discuss the possible problems and resolutions.
Masunaga, A., Liu, A. W., Tan, Y., Scott, A., Luscombe, N. M. Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica. J. Vis. Exp. (160), e61279, doi:10.3791/61279 (2020).