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Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica
Journal JoVE
Environnement
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Journal JoVE Environnement
Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica

Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica

8,530 Views

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11:55 min

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June 16, 2020

DOI:

10.3791/61279-v

DOI:
10.3791/61279-v

•

11:55 min
June 16, 2020

•

3 Views
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1Okinawa Institute of Science and Technology Graduate University, 2Francis Crick Institute, 3UCL Genetics Institute, Department of Genetics, Evolution and Environment,University College London

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Oikopleura dioica is a tunicate model organism in various fields of biology. We describe sampling methods, species identification, culturing setup, and culturing protocols for the animals and algal feed. We highlight key factors that helped strengthen the culture system and discuss the possible problems and resolutions.

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Masunaga, A., Liu, A. W., Tan, Y., Scott, A., Luscombe, N. M. Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica. J. Vis. Exp. (160), e61279, doi:10.3791/61279 (2020).

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