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January 16, 2021
DOI:
1Solid-state NMR, Leiden Institute of Chemistry, Faculty of Science,Leiden University, 2Laboratory of Biophysics,Wageningen University & Research, 3Laboratory of BioNanoTechnology,Wageningen University & Research, 4MAGNEtic resonance Facility,Wageningen University & Research, 5C.J. Gorter Center for High Field MRI, Radiology department, Leiden University Medical Centre,Leiden University, 6Institute for Medical Physics and Biophysics,Leipzig University
Summary
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Viene presentato un protocollo per studiare il tessuto biologico ad alta risoluzione spaziale utilizzando la microscopia a risonanza magnetica ad altissimo campo (MRM) utilizzando micrococchi. Vengono fornite istruzioni dettagliate per caratterizzare i microcoil. Infine, l'ottimizzazione dell'imaging è dimostrata sulle radici delle piante.
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van Schadewijk, R., Krug, J. R., Webb, A., Van As, H., Velders, A. H., de Groot, H. J. M., Alia, A. MRM Microcoil Performance Calibration and Usage Demonstrated on Medicago truncatula Roots at 22 T. J. Vis. Exp. (167), e61266, doi:10.3791/61266 (2021).
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