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使用定义的分化系统从多能干细胞的肝祖原体规范
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System
Please note that all translations are automatically generated.
Click here for the English version.
使用定义的分化系统从多能干细胞的肝祖原体规范
DOI:
10.3791/61256-v
•
07:09 min
•
May 10, 2020
•
Jose Meseguer-Ripolles
,
Yu Wang
,
Agnes Sorteberg
,
Aishwariya Sharma
,
Nan-Linda Ding
,
Baltasar Lucendo-Villarin
,
Philipp Kramer
,
Charis-Patricia Segeritz
,
David C. Hay
1
MRC Centre for Regenerative Medicine
,
University of Edinburgh
,
2
Research & Development
,
STEMCELL Technologies Inc
Chapters
00:05
Introduction
00:29
Human Pluripotent Stem Cell Seeding
03:34
Differentiating hPSCs to Hepatic Progenitors on Laminin-521
04:55
Results: Hepatic Progenitor Differentiation from hPSCs
06:26
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文的目标是提供一种标准化的方法,诱导人类肝祖细胞与多能干细胞的分化。这种程序的开发与即用媒体配方为用户提供了一个方便的系统,以产生人类肝细胞的生物医学研究和翻译。
Tags
Hepatic Progenitor
Pluripotent Stem Cells
Defined Differentiation System
Hepatic Differentiation
Liver Biology
Large-scale Production
Laminin-521
ROCK Inhibitor
Enzyme-free Dissociation
Single Cell Suspension
Viable Cell Count
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