The goal of this article is to provide a standardized approach to induce human hepatic progenitor differentiation from pluripotent stem cells. The development of this procedure with ready-to-use media formulations offer the user a facile system to generate human liver cells for biomedical research and translation.
Meseguer-Ripolles, J., Wang, Y., Sorteberg, A., Sharma, A., Ding, N., Lucendo-Villarin, B., Kramer, P., Segeritz, C., Hay, D. C. Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System. J. Vis. Exp. (159), e61256, doi:10.3791/61256 (2020).