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Hoge resolutie mapping van eiwit-DNA interacties in muis stamcel-afgeleide neuronen met behulp van chromatine immunoprecipitation-exonuclease (ChIP-Exo)
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Genetics
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JoVE Journal Genetics
High-Resolution Mapping of Protein-DNA Interactions in Mouse Stem Cell-Derived Neurons using Chromatin Immunoprecipitation-Exonuclease (ChIP-Exo)
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Hoge resolutie mapping van eiwit-DNA interacties in muis stamcel-afgeleide neuronen met behulp van chromatine immunoprecipitation-exonuclease (ChIP-Exo)

Nederlands

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4,647 Views

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08:40 min

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August 14, 2020

DOI:

10.3791/61124-v

DOI:
10.3791/61124-v

•

08:40 min
August 14, 2020

•

11 Views
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1Department of Cell and Systems Biology,University of Toronto, 2Department of Biology,University of Toronto

Summary

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Nauwkeurige bepaling van eiwitbindende locaties in het genoom is belangrijk voor het begrijpen van genregulatie. Hier beschrijven we een genomische mapping methode die chromatine-immunogeprecipiteerd DNA behandelt met exonucleasevertering (ChIP-exo) gevolgd door sequencing met hoge doorvoer. Deze methode detecteert eiwit-DNA interacties met near base-pair mapping resolutie en hoge signaal-ruisverhouding bij zoogdierneuronen.

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Cite this Article

Montanera, K. N., Rhee, H. S. High-Resolution Mapping of Protein-DNA Interactions in Mouse Stem Cell-Derived Neurons using Chromatin Immunoprecipitation-Exonuclease (ChIP-Exo). J. Vis. Exp. (162), e61124, doi:10.3791/61124 (2020).

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